FastQCFastQC Report
Thu 16 Mar 2023
H5L23AFX5_n01_37GP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L23AFX5_n01_37GP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10948876
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG280620.2563002814170149No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC244340.2231644599865776No Hit
GTCTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAAT233780.21351963434420115No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT118640.10835815475488078No Hit
ATTTATTTAAGTTTTCTTACTTAAAAAATAAATTTAACTTATAAAATTTA116780.10665935023832583No Hit
CTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAATTT114440.10452214455620834No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)