FastQCFastQC Report
Thu 16 Mar 2023
H5L23AFX5_n01_37BS.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L23AFX5_n01_37BS.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14025898
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG403810.2879031346156945No Hit
GTCTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAAT308840.2201926750073329No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC292450.20850714870448936No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT247130.17619549208186172No Hit
TTTTATTATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTTATA229740.16379699895151098No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA225030.16043892519395192No Hit
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT179630.12807023122512368No Hit
GCTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTT179530.127998934542373No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT178090.12697226231076256No Hit
ATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATC161680.11527247667136892No Hit
CTCCCAATAAAATTTTTAATTCATTAATTTTTTTATATAATAAATTAAAA158240.11281987078474405No Hit
TATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTTATAAACAAT155850.11111588006700178No Hit
GTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGTTATC152070.10842086545902443No Hit
ATTTATTTAAGTTTTCTTACTTAAAAAATAAATTTAACTTATAAAATTTA151650.1081214193914714No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)