FastQCFastQC Report
Thu 16 Mar 2023
H5L23AFX5_n01_37BB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L23AFX5_n01_37BB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17578682
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG409810.23312896837203154No Hit
GTCTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAAT359490.20450338654513459No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC346360.19703411211375232No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT277250.15771944677081023No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA270180.15369752976929668No Hit
TTTTATTATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTTATA255240.1451985990758579No Hit
ATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATC215820.12277370965582061No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT189890.10802288817785088No Hit
ATTTATTTAAGTTTTCTTACTTAAAAAATAAATTTAACTTATAAAATTTA185410.10547434671154528No Hit
TATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTTATAAACAAT181420.10320455196811684No Hit
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT177820.10115661686126412No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)