FastQCFastQC Report
Thu 16 Mar 2023
H5L23AFX5_n01_33SS.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L23AFX5_n01_33SS.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11983060
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG211390.17640736172563604No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC160090.1335969276628841No Hit
ATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATC149090.12441730242525699No Hit
GTCTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAAT148920.12427543548976638No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT137330.11460344853484836No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT127920.10675069639975099No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA127390.10630840536557441No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)