FastQCFastQC Report
Thu 16 Mar 2023
H5L23AFX5_n01_33GP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5L23AFX5_n01_33GP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12288233
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG240670.19585403369223225No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC183310.14917523129647686No Hit
GTCTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAAT171720.13974344399231362No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT161340.1312963385378516No Hit
ATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATC137450.11185497540614668No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA129570.10544233658329884No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT128920.10491337525907916No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)