FastQCFastQC Report
Fri 12 May 2023
H5KKWDRX3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5KKWDRX3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences943987821
Sequences flagged as poor quality0
Sequence length10
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTAACACT61002733264.62237313123131No Hit
TACCGCTTAT20814891422.049957570374204No Hit
AGGTAACACT797802898.451410836581122No Hit
CGATCTCGGT210978392.234969406453815No Hit
AGATCTCGGT26566870.28143233852166405Illumina Single End PCR Primer 1 (100% over 10bp)
TGGTAACACT21825450.23120478373205622No Hit
CGTTCTCGGT19389470.20539957792527494No Hit
NGGTAACACT15841560.16781530065947747No Hit
GGGGGGGGGG12550490.1329518212078713No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)