FastQCFastQC Report
Tue 18 Dec 2018
H5K5JAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5K5JAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6893074
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2828794.103814930755132No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110930.16092965199561182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACTC10450.032.0809942
ACCTTAG12400.031.8426729
CCTTAGA13150.030.5635710
CTTAGAG12900.030.34290511
ACTCTAG10250.029.9429445
CTCTAGT10050.029.5020266
TCCACCT13650.029.3876026
TTGTACT10750.029.0354461
CACCTTA14000.028.696618
CTATCCC4750.028.0567231
TTCCACC14550.027.8054985
ACACGAG14550.027.64070528
GAGTACA15250.027.52117215
GTACTCT11950.027.1494773
TTAGAGT14900.026.99675212
TAGAGTA14850.026.85367413
CCACCTT15050.026.679927
TATCCCC4950.026.1029382
AGAGTAC16750.025.26465614
TCTAGTT11950.025.1277587
Produced by FastQC (version 0.11.5)