FastQCFastQC Report
Tue 18 Dec 2018
H5K5JAFXY_n02_sim_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5K5JAFXY_n02_sim_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16050997
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGT36450.020.93231411
CTAGCAC36050.020.19390762
GATAGTT37700.020.05265812
CACTAGC39300.018.435160
ACGATAG43400.018.06421910
GCCACTA41900.017.70881758
ACTCTAG11650.016.8234445
ACTAGCA45200.016.26084761
TAGCACA47250.015.85154263
GCCACCA49150.015.09818337
CGCCACT51800.014.52701657
ATAGTTT54950.014.5220113
CGCCACC50950.014.35867736
AGGTGGG26450.014.29083712
CTAGTGG19350.014.28961669
CACCACA52650.014.22720639
CCACCAC52800.013.7229938
TACCCCC13200.012.46183911
CCACTAG60300.012.18908359
GCAGAAT84700.012.1484851
Produced by FastQC (version 0.11.5)