FastQCFastQC Report
Tue 18 Dec 2018
H5K5JAFXY_n02_sim_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5K5JAFXY_n02_sim_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15875219
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAG37250.023.8649041
ACTCTAG16200.021.8199885
GGGGAGG43100.018.8391632
AGGTAGC43300.018.3479486
GGTAGCG44900.017.8503027
GGAGGTA45900.017.6133524
GGGAGGT49500.016.6860543
CTCTAGT21700.016.1283456
TACTCTA25250.016.078274
GAGGTAG51150.015.5320375
GGTACCA22450.014.965724
ACCTTAG21800.014.1281519
GGTGCCC60000.013.06652213
TCTAGTT31700.012.5864137
TAGCGGT68650.012.3886759
GCGGGGA42450.012.2021481
ACCTCTA28200.012.0387617
ATTCGAC73250.011.8970685
GTGCCCT68750.011.75975814
TACCACC25700.011.7115636
Produced by FastQC (version 0.11.5)