FastQCFastQC Report
Tue 18 Dec 2018
H5K5JAFXY_n02_sim_16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5K5JAFXY_n02_sim_16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14312244
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG15450.023.5587465
GATAGTT36750.023.23796312
CGATAGT37200.023.05070111
CTAGCAC37700.022.55955162
CACTAGC40250.020.7825660
ACTAGCA43250.019.58363361
ACGATAG44500.019.50536710
GCCACTA45700.018.84015358
GCCACCA44650.018.73658837
CGCCACC45700.018.45928836
TAGCACA46550.018.34575863
CGGGGAG31300.017.5551951
CACCACA49050.017.41277339
ATAGTTT50800.017.36210413
AGGTGGG23500.017.27649712
CCACCAC52350.016.85015938
AGGTAGC29650.016.5254066
CGCCACT51450.016.25835257
GGTAGCG28150.016.0385917
TACCCCC16350.015.626657511
Produced by FastQC (version 0.11.5)