FastQCFastQC Report
Tue 18 Dec 2018
H5K5JAFXY_n02_1g_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5K5JAFXY_n02_1g_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15089191
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG13700.019.4148525
TACCCCC14800.017.49972511
CTAGCAC29250.016.03398962
CGGGGAG26800.015.9317931
AGGTGGG25800.015.46502712
CACTAGC31100.014.85521960
CGATAGT31200.014.8074611
ACCTTAG19400.014.7934849
GATAGTT32000.014.76553912
GTCTAAC19750.014.3534921
CTAGTGG21150.013.73541869
ACCCTAC19250.013.45531542
CTTACCC19400.013.3502179
ACGATAG37900.013.11316410
GGGAGTG32050.013.10440517
GCCACTA35350.012.87134258
ACCCCCA20450.012.83611212
GTGGGGA32350.012.76641414
ACTAGCA36600.012.7183861
GGGGAGT33050.012.39016416
Produced by FastQC (version 0.11.5)