FastQCFastQC Report
Tue 18 Dec 2018
H5K5JAFXY_n02_1g_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5K5JAFXY_n02_1g_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19361664
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAG2379511.2289801124531445No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1021680.5276819182483489No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA520280.26871657312098796No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGT391070.2019816065396032No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA341210.17622968769626413No Hit
ACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAG202270.10446932660333327No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT200510.10356031382426634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTAG513700.045.362929
TTCCACC524100.045.2286385
TCCACCT525150.045.0588076
CACCTTA528700.044.5393038
TTTCCAC541900.043.917034
CCACCTT542200.043.572137
CCTTAGA540200.042.99505610
TTGTACT266250.042.5633741
CTTAGAG542650.042.5429511
TGTACTC275300.041.08792
TAGAGTA569700.040.5907513
ACTCTAG278700.040.146925
TTAGAGT585650.039.52102712
AGAGTAC585400.039.4533914
TTTTCCA626500.038.1880533
AGTACAA616800.037.78545416
GAGTACA612900.037.69458815
CTCTAGT300600.037.245816
TACTCTA303700.037.245154
GTACTCT307550.036.699533