FastQCFastQC Report
Tue 18 Dec 2018
H5K5JAFXY_n02_1g_16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5K5JAFXY_n02_1g_16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14607574
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG21300.032.3699875
ACCTTAG29800.026.0737239
CTCTAGT30700.022.3446546
CACCTTA40300.019.888268
CCTTAGA39500.019.7595510
CGGGGAG34800.019.3096081
CTAGTTG39000.019.0255668
CGATAGT33850.019.02503611
CTTAGAG43350.018.24675811
GGTAGCG28650.018.2023727
GATAGTT34800.018.10350212
ACCCTAC38700.017.99839242
AGGTAGC29400.017.9756896
TGTACTC40150.017.6954652
TCTAGTT43400.017.2579987
CTAGATT38600.017.13735657
CACTAGC36650.016.99851260
CTAGCAC36450.016.99558662
TAACCTA39800.016.97200453
ACACGAG45550.016.90414628
Produced by FastQC (version 0.11.5)