FastQCFastQC Report
Tue 18 Dec 2018
H5K5JAFXY_n02_1g_0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5K5JAFXY_n02_1g_0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23088685
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCATATCAACTGAAAAATGAGGTGTTTGTCAAAGACGTCTTAGAAACAG990760.42911062280073553No Hit
CTTTGGTAAGCACCTCATTTGTCAATAAAATGAAGACTTGTTTAATGCCT989470.4285519075685774No Hit
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAG885280.38342590753869266No Hit
TATCAACTGAAAAATGAGGTGTTTGTCAAAGACGTCTTAGAAACAGTTGG796640.34503480817551974No Hit
GTTTGTCAAAGACGTCTTAGAAACAGTTGGATAGAAAATAAACATGTCCA762260.33014439756963204No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT692160.2997832054965452No Hit
CTTATATGAAGAAAATGTGTTATGCCTTTGGTAAGCACCTCATTTGTCAA664890.287972225356273No Hit
AAAGCATATCAACTGAAAAATGAGGTGTTTGTCAAAGACGTCTTAGAAAC611540.2648656690495799No Hit
GCCTTTGGTAAGCACCTCATTTGTCAATAAAATGAAGACTTGTTTAATGC603890.2615523577891075No Hit
TGAAAAATGAGGTGTTTGTCAAAGACGTCTTAGAAACAGTTGGATAGAAA551480.23885292731049865No Hit
GCTTATATGAAGAAAATGTGTTATGCCTTTGGTAAGCACCTCATTTGTCA530340.22969692730443503No Hit
GTCAAAGACGTCTTAGAAACAGTTGGATAGAAAATAAACATGTCCAATGC429550.18604351005698244No Hit
AAGCATATCAACTGAAAAATGAGGTGTTTGTCAAAGACGTCTTAGAAACA424950.18405119217486834No Hit
CTGGTTTTGACAGCAAGTCGAATTGAAAAGTAAGCACGTCGAGCATTGAG421610.18260459614742028No Hit
ATCAACTGAAAAATGAGGTGTTTGTCAAAGACGTCTTAGAAACAGTTGGA403270.1746613113739479No Hit
CAAAGACGTCTTAGAAACAGTTGGATAGAAAATAAACATGTCCAATGCTC398740.17269931137264855No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA340000.1472582782432174No Hit
CCTTTGGTAAGCACCTCATTTGTCAATAAAATGAAGACTTGTTTAATGCC290690.1259014967721202No Hit
CTTGTTTAATGCCTAACGATTCCTGCGATAAACACCTCATTTGACAATTT283100.12261417226663189No Hit
CTGAAAAATGAGGTGTTTGTCAAAGACGTCTTAGAAACAGTTGGATAGAA280810.12162234445140553No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGT279520.12106362921924743No Hit
GTTATGCCTTTGGTAAGCACCTCATTTGTCAATAAAATGAAGACTTGTTT273620.11850826497914456No Hit
GCATATCAACTGAAAAATGAGGTGTTTGTCAAAGACGTCTTAGAAACAGT272350.11795821199864782No Hit
AGAAAATGTGTTATGCCTTTGGTAAGCACCTCATTTGTCAATAAAATGAA267320.11577965570581433No Hit
CTTAGAAACAGTTGGATAGAAAATAAACATGTCCAATGCTCAAACCATTT259040.11219348351800894No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATC255220.11053899345068807Illumina Single End PCR Primer 1 (96% over 33bp)
GTTGGATAGAAAATAAACATGTCCAATGCTCAAACCATTTCTCTTCACTG247290.10710441066695657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACTC185850.041.2788622
CTCTAGT219450.034.7670446
TTGTACT222950.034.5669021
GTACTCT222750.034.487433
TACTCTA231900.033.23214
GTAGGGA71750.032.87751419
TGTGTAG67750.032.0835141
CACCTTA263900.031.405058
TTTCCAC270400.031.0761134
AGAGTGT73850.030.56802427
AGATCTC69000.029.97764246
CCTTAGA277050.029.6240710
TCCACCT282250.029.5362036
TCTCGGT71400.029.41058549
ACTCTAG288950.029.2266255
CTGTGTA77250.029.13498740
CTAGTTG260800.028.8796488
GAGTGTC76300.028.80693228
CTTAGAG283400.028.76257911
GTCGTGT84250.028.20713414
Produced by FastQC (version 0.11.5)