FastQCFastQC Report
Tue 18 Dec 2018
H5K5JAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5K5JAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6893074
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3448735.003181454311966No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA115080.16695018797128827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT25750.039.18212514
ACACGTC26400.038.08480513
GAACTCC25700.038.06347721
CACACGT26650.037.47052412
TGAACTC26700.037.02069520
ACGTCTG27050.036.784615
GCACACG27450.036.50815611
CTCCAGT24800.036.393624
AGCACAC30700.033.54614610
AACTCCA29400.033.3901322
CGTCTGA30200.032.9470816
AGAGCAC31250.032.7304468
CTGAACT30900.032.32150319
CCAGTCA23600.030.89454726
TATCTCG10750.030.44358441
CAGTCAC22400.030.37743427
TCGGAAG36650.029.0906073
CCGGGGT7350.028.5842061
CAGTCTA4600.027.30921227
CTCGTAT16200.027.1345844
Produced by FastQC (version 0.11.5)