FastQCFastQC Report
Tue 18 Dec 2018
H5K5JAFXY_n01_sim_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5K5JAFXY_n01_sim_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16050997
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT350240.21820451402489202TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG66650.040.22477341
AGTCACG72100.039.32023628
GAACTCC73850.038.5779321
CTCCAGT79150.036.3042324
ACACGTC80100.035.6114913
ACTCCAG83300.034.3274923
CACACGT86000.033.24976712
GTATGCC86700.032.0532347
CACGTCT90250.031.64520614
TCGTATG88350.030.86028745
CGTATGC91750.030.21260346
GCACACG95500.030.19873811
CCAGTCA96500.030.03087826
GTCACGA96650.029.91193829
CAGTCAC98200.029.68929327
TGAACTC96150.029.66697720
ATTCGTA96200.029.57879336
TCCAGTC97950.029.4791225
CTCGTAT92650.029.35219644
CGTATCT92750.029.28281239
Produced by FastQC (version 0.11.5)