FastQCFastQC Report
Tue 18 Dec 2018
H5K5JAFXY_n01_sim_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5K5JAFXY_n01_sim_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15875219
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAG31600.021.046261
GGGGAGG28950.020.9153392
ACACGTC36600.020.17750213
GAACTCC40500.018.75288221
ACTCTAG13600.017.757275
CACGTCT47100.015.38214314
GGTAGCG38450.015.2924197
AGTCACA48500.015.22692428
GGAGGTA41250.014.9332644
AGGTAGC38400.014.8567016
CTCCAGT49350.014.7517524
AGCACAC52500.014.66659710
CACACGT52150.014.49657712
ACTCCAG54750.013.9998923
GGGAGGT44250.013.6835523
GGTACCA23100.013.63634
GAGGTAG44500.013.6066785
AATCTCG47600.013.52934741
TGAACTC62150.012.44559920
CTCTAGT20850.012.4220046
Produced by FastQC (version 0.11.5)