FastQCFastQC Report
Tue 18 Dec 2018
H5K5JAFXY_n01_sim_16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5K5JAFXY_n01_sim_16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14312244
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT407120.28445574292892156TruSeq Adapter, Index 19 (97% over 38bp)
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA143300.10012406160766962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCTC59500.050.70543340
GCTATCT63300.047.77208739
TATCTCG69550.043.5294541
AGTCACC76800.041.60813528
ACTCCAG82000.039.01198223
CTCCAGT82900.038.54623424
AGCTATC79150.038.42669738
TCGTATG85250.037.6890845
GAAGCTA85400.037.4996736
CACACGT87400.037.04213312
GTCACCT86450.036.96362329
GTATGCC88550.036.24498747
GAACTCC90000.035.77746221
AACTCCA92600.034.73511522
CACGTCT94600.034.14874314
CAGTCAC96000.034.0156827
CTCGTAT91200.033.96339444
CGTATGC95700.033.57360546
ACACGTC97050.033.53911613
GCACACG98450.033.02673711
Produced by FastQC (version 0.11.5)