FastQCFastQC Report
Tue 18 Dec 2018
H5K5JAFXY_n01_1g_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5K5JAFXY_n01_1g_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15089191
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCTC30450.027.93063240
GCTATCT33350.026.34144839
TATCTCG39500.021.8857641
GAACTCC43600.021.51359221
ACACGTC44400.021.44113213
AGTCACC45750.020.50277528
AGGTGGG23250.020.32235312
CTCCAGT48850.019.273224
CACACGT50150.018.8432612
ACTCCAG50150.018.77359423
CACGTCT52500.018.19973814
GGTGGGG26800.017.63034213
AGCTATC51200.017.08959638
GTCACCT56750.017.08373529
CTAGCAC29400.017.02367862
GCACACG58700.016.81412111
GTGGGGA29000.016.7756214
GAAGCTA55450.016.53723536
GTATGCC55250.016.47089647
CACTAGC31150.016.29200660
Produced by FastQC (version 0.11.5)