H5K5JAFXY_n01_1g_4.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H5K5JAFXY_n01_1g_4.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19361664
Sequences flagged as poor quality	0
Sequence length	76
%GC	43

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT	191745	0.9903332688760637	TruSeq Adapter, Index 7 (97% over 35bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA	90200	0.4658690492717981	No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT	54981	0.2839683613970369	No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGT	54158	0.279717693685832	No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT	38738	0.20007577861076406	No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA	36850	0.19032455061713704	No Hit
ACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAG	29594	0.1528484328619689	No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA	22905	0.1183007824120902	No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG	21910	0.11316176130316072	No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA	21477	0.11092538327284267	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
AGTCACG	27320	56.766426	28
TATCTCG	25740	54.824352	41
GAACTCC	28835	54.183475	21
GTATGCC	28095	52.92088	47
CTCGTAT	26765	52.89505	44
CTCCAGT	29765	52.385242	24
GTCACGA	29790	52.142086	29
CAGTCAC	29895	52.12259	27
CGTATGC	28315	51.990536	46
ACACGTC	30440	51.533257	13
TCGTATG	28495	51.440758	45
CGTATCT	27940	51.30931	39
CCAGTCA	30450	51.11523	26
CACGTCT	30655	51.114742	14
TCCAGTC	30690	50.703968	25
GTATCTC	28075	50.563797	40
TATGCCG	29430	50.25917	48
ACTCCAG	31305	49.852825	23
TGAACTC	31640	49.435097	20
ATTCGTA	31725	48.64097	36