FastQCFastQC Report
Tue 18 Dec 2018
H5K5JAFXY_n01_1g_16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5K5JAFXY_n01_1g_16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14607574
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT358020.24509203239360622TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACTA71800.050.69653329
CACTAAT73650.049.5656631
TCACTAA85600.042.68704630
ACTCCAG88700.041.31283623
CACACGT90100.040.98166312
CTCCAGT89600.040.66362824
ACTAATG91600.040.19648732
ATGCGCA91750.039.32914736
GAACTCC94850.038.44964221
TAATGCG95400.038.3013534
CACGTCT96150.038.22112314
TGCGCAT93050.037.68888537
AGTCACT99200.037.1876128
CAGTCAC99800.037.1043227
GCACACG99900.037.0315111
ACACGTC100300.036.81403713
AACTCCA101050.036.19444322
TGAACTC101200.036.03716720
CATCTCG76050.035.7588741
TCCAGTC104650.035.21713625
Produced by FastQC (version 0.11.5)