FastQCFastQC Report
Tue 18 Dec 2018
H5K5JAFXY_n01_1g_0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5K5JAFXY_n01_1g_0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23088685
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT1312000.5682437089855918TruSeq Adapter, Index 13 (97% over 38bp)
AGCATATCAACTGAAAAATGAGGTGTTTGTCAAAGACGTCTTAGAAACAG1024690.44380613274424247No Hit
CTTTGGTAAGCACCTCATTTGTCAATAAAATGAAGACTTGTTTAATGCCT992260.4297602916753379No Hit
TATCAACTGAAAAATGAGGTGTTTGTCAAAGACGTCTTAGAAACAGTTGG815340.35313401347889667No Hit
GTTTGTCAAAGACGTCTTAGAAACAGTTGGATAGAAAATAAACATGTCCA745780.3230067022006667No Hit
CTTATATGAAGAAAATGTGTTATGCCTTTGGTAAGCACCTCATTTGTCAA682970.29580290085814764No Hit
AAAGCATATCAACTGAAAAATGAGGTGTTTGTCAAAGACGTCTTAGAAAC645270.27947455647647323No Hit
GCCTTTGGTAAGCACCTCATTTGTCAATAAAATGAAGACTTGTTTAATGC607500.263115894213984No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA582570.25231839751809165No Hit
TGAAAAATGAGGTGTTTGTCAAAGACGTCTTAGAAACAGTTGGATAGAAA567450.24576973526209914No Hit
GCTTATATGAAGAAAATGTGTTATGCCTTTGGTAAGCACCTCATTTGTCA545510.23626724518958095No Hit
AAGCATATCAACTGAAAAATGAGGTGTTTGTCAAAGACGTCTTAGAAACA437710.1895777087348197No Hit
GTCAAAGACGTCTTAGAAACAGTTGGATAGAAAATAAACATGTCCAATGC435370.1885642252904399No Hit
CTGGTTTTGACAGCAAGTCGAATTGAAAAGTAAGCACGTCGAGCATTGAG421090.18237937760422476No Hit
CAAAGACGTCTTAGAAACAGTTGGATAGAAAATAAACATGTCCAATGCTC420720.1822191259484895No Hit
ATCAACTGAAAAATGAGGTGTTTGTCAAAGACGTCTTAGAAACAGTTGGA414610.17957280806594225No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT361000.1563536424876514No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGT306740.1328529537303662No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT305650.13238086101482177No Hit
CCTTTGGTAAGCACCTCATTTGTCAATAAAATGAAGACTTGTTTAATGCC304380.13183080803432504No Hit
CTGAAAAATGAGGTGTTTGTCAAAGACGTCTTAGAAACAGTTGGATAGAA289110.1252171788908723No Hit
CTTGTTTAATGCCTAACGATTCCTGCGATAAACACCTCATTTGACAATTT287680.12459782789708465No Hit
GCATATCAACTGAAAAATGAGGTGTTTGTCAAAGACGTCTTAGAAACAGT286950.1242816557114448No Hit
GTTATGCCTTTGGTAAGCACCTCATTTGTCAATAAAATGAAGACTTGTTT277810.12032300670220067No Hit
AGAAAATGTGTTATGCCTTTGGTAAGCACCTCATTTGTCAATAAAATGAA276560.11978161597336531No Hit
CTTAGAAACAGTTGGATAGAAAATAAACATGTCCAATGCTCAAACCATTT269930.11691007954762256No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTA259690.11247500669700332TruSeq Adapter, Index 13 (97% over 38bp)
CTCATTTGTCAATAAAATGAAGACTTGTTTAATGCCTAACGATTCCTGCG236930.10261736430636911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACA232950.046.4866328
GTCACAT233750.046.2826129
CAGTCAC234350.046.1939927
CTCGTAT208150.045.5844844
AATCTCG208000.045.583641
CACATTC237350.045.46245631
CACACGT246700.044.34918612
GTATGCC231250.044.2707147
GCCGTCT223250.044.03917751
ACACGTC252200.043.47896613
TCACATT252950.042.8387530
TCCAGTC252950.042.75572625
CACGTCT257800.042.60238614
GCACACG257900.042.5589111
AGCACAC257550.042.52152610
TGAACTC255950.042.5000820
TCGTATG240100.041.9972345
CGTCTGA263050.041.6722916
CTCCAGT261150.041.6676824
CGTATGC245950.041.5107946
Produced by FastQC (version 0.11.5)