H5JYVAFXY_n02_wildtype-2_gp.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H5JYVAFXY_n02_wildtype-2_gp.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3179650
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	4286	0.13479471010960326	No Hit
GTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA	3695	0.11620775871558191	Illumina Single End PCR Primer 1 (100% over 36bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	3594	0.11303130847734814	Illumina Single End PCR Primer 1 (100% over 51bp)

Adapter graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCCC	520	0.0	44.148464	45
GTCGCCG	1180	0.0	41.199364	45
CCGCCAG	35	6.2006766E-6	38.61757	2
GTCGGCC	25	0.002104425	36.011295	45
GGTCGCC	1995	0.0	34.181503	44
CTCGCCG	55	1.1256816E-7	32.737537	45
CGGTGGG	2925	0.0	32.31783	45
TTCGCCG	65	1.4009856E-8	31.163622	45
GCTCGCC	75	1.7007551E-9	30.007523	44
GTAGCCC	55	4.157817E-6	28.645346	45
CGGGCCA	80	3.3487595E-9	28.164856	1
CCGAGAT	265	0.0	28.048407	4
GGCGCCG	105	3.6379788E-12	27.865885	45
GTAGCCG	100	4.5474735E-11	27.00847	45
TGTCGCC	125	0.0	27.006771	44
CGGGGCA	110	5.456968E-12	26.628588	1
GTGGTCC	285	0.0	25.271086	45
GTGGCCG	170	0.0	25.15495	45
CCGCGCA	45	0.0012169441	25.035427	1
CCGGGCA	55	1.3558513E-4	24.580235	1