Basic Statistics
Measure | Value |
---|---|
Filename | H5JYVAFXY_n02_wildtype-2_gp_b.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 3359842 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 52 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 5228 | 0.1556025551201515 | Illumina Single End PCR Primer 1 (100% over 51bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 4642 | 0.13816125877347804 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CCGGGCA | 40 | 6.7539077E-9 | 45.05126 | 1 |
ACGCGCA | 35 | 1.202061E-7 | 45.051258 | 1 |
GTCCCCC | 45 | 3.8562575E-10 | 45.005642 | 45 |
GTCGCCC | 605 | 0.0 | 44.26175 | 45 |
GTAGCCC | 80 | 0.0 | 42.19279 | 45 |
GTCGCCG | 1720 | 0.0 | 42.127377 | 45 |
TGGCGCA | 75 | 0.0 | 42.047844 | 1 |
TTGCGCA | 50 | 1.0713848E-9 | 40.54613 | 1 |
CGGGGCA | 140 | 0.0 | 40.22434 | 1 |
CGCCGCA | 45 | 1.9108484E-8 | 40.045567 | 1 |
GCGCCGA | 30 | 1.1327724E-4 | 37.54272 | 1 |
CAGTTCG | 105 | 0.0 | 36.47007 | 1 |
AGCCGGA | 75 | 1.8189894E-12 | 36.041008 | 1 |
CCGGTTA | 25 | 0.0020959405 | 36.041008 | 1 |
GTTAGCC | 25 | 0.0021070032 | 36.00237 | 44 |
CGGATCG | 445 | 0.0 | 35.43358 | 1 |
GCCTCAG | 90 | 0.0 | 35.036736 | 2 |
CGGTGGG | 5255 | 0.0 | 34.55714 | 45 |
CCGTGGG | 235 | 0.0 | 34.47241 | 45 |
CGGGCAA | 105 | 0.0 | 34.324768 | 1 |