FastQCFastQC Report
Fri 21 Dec 2018
H5JYVAFXY_n02_wildtype-2_gp_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JYVAFXY_n02_wildtype-2_gp_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3359842
Sequences flagged as poor quality0
Sequence length51
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG52280.1556025551201515Illumina Single End PCR Primer 1 (100% over 51bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46420.13816125877347804No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGCA406.7539077E-945.051261
ACGCGCA351.202061E-745.0512581
GTCCCCC453.8562575E-1045.00564245
GTCGCCC6050.044.2617545
GTAGCCC800.042.1927945
GTCGCCG17200.042.12737745
TGGCGCA750.042.0478441
TTGCGCA501.0713848E-940.546131
CGGGGCA1400.040.224341
CGCCGCA451.9108484E-840.0455671
GCGCCGA301.1327724E-437.542721
CAGTTCG1050.036.470071
AGCCGGA751.8189894E-1236.0410081
CCGGTTA250.002095940536.0410081
GTTAGCC250.002107003236.0023744
CGGATCG4450.035.433581
GCCTCAG900.035.0367362
CGGTGGG52550.034.5571445
CCGTGGG2350.034.4724145
CGGGCAA1050.034.3247681