FastQCFastQC Report
Fri 21 Dec 2018
H5JYVAFXY_n02_wildtype-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JYVAFXY_n02_wildtype-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3489423
Sequences flagged as poor quality0
Sequence length51
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58260.1669617011179212No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG41500.11893083756254258Illumina Single End PCR Primer 1 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGCA253.8682047E-545.0476261
GTCGCCC3350.042.98923545
GTCGCCG17250.042.13451845
CCGCCCA451.9123036E-840.0423361
TCGCGCA1350.038.3739051
CGGGGCA2650.038.2479861
CCGCGCA659.094947E-1238.117221
CGGCCCA850.037.0980451
CGGCGCA1800.036.2883641
GGTCGCC23100.035.55284544
CGGTGGG159300.035.51191345
TTCGCCG702.1827873E-1135.36056545
CGGCCTA653.6379788E-1034.652021
TCCGGCA606.373739E-933.785721
TGCCCGA401.5502084E-533.785721
ACCGGCA606.373739E-933.785721
CGGCCAA1200.033.785721
CCGGCAA606.373739E-933.785721
ACGCGCA801.8189894E-1233.785721
CGTCGCC401.5612053E-533.7508544
Produced by FastQC (version 0.11.5)