FastQCFastQC Report
Fri 21 Dec 2018
H5JYVAFXY_n02_wildtype-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JYVAFXY_n02_wildtype-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2347222
Sequences flagged as poor quality0
Sequence length51
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG39510.16832664315518514Illumina Single End PCR Primer 1 (100% over 51bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29530.12580829593451323No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCCA406.7575456E-945.0468441
GGCTCAG750.045.042042
GTCGCCC4300.044.48508545
GTAGCCC700.041.79355245
GTCGCCG12000.041.6328145
TGGCGCA650.041.5817031
CGAGCCA501.0713848E-940.5421641
CGGCCTA451.9121217E-840.041641
CCGGGGA403.4321602E-739.415991
ACGCACA403.4321602E-739.415991
GTCCCAG403.4350342E-739.4117852
TCGCGCA700.038.611581
CAGCGCA301.1333214E-437.539041
TTGGCCA301.1333214E-437.539041
TGGCCCA301.1333214E-437.539041
CGGGGCA2100.037.539041
TGCGCTA301.1333214E-437.539041
TGCGCCA301.1333214E-437.539041
TGTCGCC1750.037.29112244
CGAGGCA850.037.0974041