FastQCFastQC Report
Fri 21 Dec 2018
H5JYVAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JYVAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14748602
Sequences flagged as poor quality0
Sequence length51
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2646231.794224293258439No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCC12000.037.88722245
GTCGCCG35100.035.5242845
GGTCGCC49100.032.77300344
CGGTGGG231700.030.97783745
CGGGCCA3550.026.032121
TGGTCGC61050.024.91975843
GCACACG9200.024.68442218
CCGTGGG13600.024.16220545
CGGTGGT372350.023.74943545
TCTCGGG174100.022.7895843
TCTCGGT797100.021.82193843
CACGGTG35700.021.74916344
ATCTCGG939650.021.71005442
CTCGGTG829850.021.67984844
GATCTCG924500.021.66713741
CTCGGGG216100.021.14136144
TCGGTGG961350.021.0990245
AGATCTC999000.020.96467840
CCCAGAT22600.020.9374944
CCAGATC93800.020.8978775
Produced by FastQC (version 0.11.5)