FastQCFastQC Report
Fri 21 Dec 2018
H5JYVAFXY_n02_rnh202_pol2-MG-2_gp.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JYVAFXY_n02_rnh202_pol2-MG-2_gp.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3393327
Sequences flagged as poor quality0
Sequence length51
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49070.14460734258737812No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG45680.1346171471243414Illumina Single End PCR Primer 1 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCC6550.041.91166345
GTCGCCG14350.041.0833445
GGTCGCC23900.034.08114644
CGGATCG5400.032.5380331
GTCGCGC300.005146696730.00233845
TGGTCGC27650.028.39850243
CGGTGGG28900.028.34130945
CGGGGCA1600.026.7500131
CGGGGCT450.001218711425.0292551
GTCCCCC450.001226490825.0019545
GGCGCCC450.001226490825.0019545
CTGATCG2450.023.9054951
GGGCGCC2300.022.50109144
TGTCGCC1800.022.50109144
GGGTCGC3250.022.15296243
TCCTGCC1156.7939254E-921.51802438
GTAGCCG1056.1116225E-821.43024345
GTGGCCG1900.021.31745145
CCTGCCG955.545935E-721.31273839
TGGGGCC1301.2114469E-920.7935331
Produced by FastQC (version 0.11.5)