FastQCFastQC Report
Fri 21 Dec 2018
H5JYVAFXY_n02_rnh202_pol2-MG-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JYVAFXY_n02_rnh202_pol2-MG-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3440865
Sequences flagged as poor quality0
Sequence length51
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG49850.14487636103131044Illumina Single End PCR Primer 1 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCC6450.043.2616745
GTCGCCG15900.040.76023545
GGGCCGA552.7212081E-936.860681
TGCCGCG250.002107343636.0012142
CGGCCCA458.6284126E-735.0403981
CTCGCCC458.697425E-735.00473845
GGTCGCC25300.034.50909444
CGGTGGG35500.033.08898545
CGGGGCA1500.033.0380941
GTCCCCG551.1274824E-732.731745
GTCCCCC551.1274824E-732.731745
GGTCCCC950.030.792344
TGGTCGC29550.029.54499843
CCGCAGA1950.028.8714663
CCTGCCG654.881058E-727.69042239
CCCGGCA507.106459E-527.0311661
CAATCGG1750.027.0311641
GTAGCCC1250.027.00365445
GGTAGCC2200.026.5933544
CCGTGGG2550.026.47417345
Produced by FastQC (version 0.11.5)