FastQCFastQC Report
Fri 21 Dec 2018
H5JYVAFXY_n02_rnh202_pol2-MG-1_gp.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JYVAFXY_n02_rnh202_pol2-MG-1_gp.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1973975
Sequences flagged as poor quality0
Sequence length51
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25420.1287756937144594No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG25130.12730657683101357Illumina Single End PCR Primer 1 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCC3800.042.63722245
GTCGCCG9600.042.19308545
CGGGGCA1050.038.6107831
GTAGCCC504.8763468E-836.00476545
CGGTGGG37900.033.24978345
GGTCGCC16750.032.64528344
GTGGTCC2850.032.37270445
CGGCGCA602.3989378E-730.0306071
GGCCCGA300.00512253730.0306071
TCGGCCA300.00512253730.0306071
CCGTGGG2000.029.25387245
CGGATCG2400.029.092151
CTCGCCG554.1626354E-628.64015445
GCCCGAG406.132869E-428.1522662
GGCGCAG2200.026.616692
CGGTTGG1300.025.96497345
CCCGAGA1058.367351E-1125.7372573
GGTCGAC801.0535041E-725.31520744
CGCTGGG1251.8189894E-1225.20333545
GTCGACC450.001225920625.00330745
Produced by FastQC (version 0.11.5)