FastQCFastQC Report
Fri 21 Dec 2018
H5JYVAFXY_n02_rnh202_pol2-MG-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JYVAFXY_n02_rnh202_pol2-MG-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1484749
Sequences flagged as poor quality0
Sequence length51
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28040.18885346950898774No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG19690.13261500765449244Illumina Single End PCR Primer 1 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCC1550.042.0985145
GTCGCCG7700.041.78743445
GGTCGCC11100.034.66242244
CGGTGGG28900.031.68815445
TGGGGCA1850.030.429211
GTGGCCG1200.030.00123245
TCGCCGG406.165513E-428.1261645
TGGTCGC12750.027.7060943
GGGCGCC1150.027.3915144
CGCGCAG1004.5474735E-1127.0202272
GGTGGCC1700.026.47078744
CGGGGCA801.0462645E-725.332321
CCGGGCA450.00122113925.0195731
GGCCGAT450.0012228225.013674
GTAGCCC551.3680822E-424.54646745
CTCGGGG27850.024.15637444
TCTCGGG24750.024.0910943
TGCGGGA1052.3464963E-923.5898841
GCGCAGA2950.022.894643
CCAGATC10500.022.725945
Produced by FastQC (version 0.11.5)