FastQCFastQC Report
Fri 21 Dec 2018
H5JYVAFXY_n02_ds774-041-2_ctrl.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JYVAFXY_n02_ds774-041-2_ctrl.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8352289
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1649701.9751471722302714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC20900.036.6051844
GTCGCCG17900.032.81090545
GTGGTCG26700.029.49429942
GTCGCCC8300.024.40030145
CTCGGTG23450.023.31159238
GGTGGTC33600.023.16902441
CGGTGGT32200.022.14896640
TGGTCGC36150.020.78907243
TCGGTGG32450.020.45216239
TCTCGGT25900.020.32374637
ATCTCGG26550.019.82570536
CAGATCG47950.017.705636
GATCTCG32400.017.1482135
AGAGATC55350.017.14795317
GAGCACA25600.016.4299916
GCACACG8850.016.2644218
CCGGGTG4300.016.2401431
AGAGCAC27150.015.82442115
GCGGGTT6200.015.6233221
TAGATCG48900.015.0590476
Produced by FastQC (version 0.11.5)