FastQCFastQC Report
Fri 21 Dec 2018
H5JYVAFXY_n02_Clb2-202-TAP-2_gp.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JYVAFXY_n02_Clb2-202-TAP-2_gp.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1802495
Sequences flagged as poor quality0
Sequence length51
%GC63

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33100.18363435127420602No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA302.1520518E-645.0428281
CAGGACA207.0014666E-445.0428281
TGCAGCA207.0014666E-445.0428281
TCCCAGA253.872431E-545.0378233
GGCGCCC253.8915216E-545.0003345
TTTCGGG207.035059E-444.99908443
GGTTTCG253.894711E-544.9940941
GTCGCCC556.184564E-1140.90939345
GTCGCCG3650.040.06878745
GCCATAG403.4369623E-739.4080962
CCCGAGA356.214319E-638.6038483
CTCACAG301.1338128E-437.535691
CAGTTCG301.1338128E-437.535691
CAGGCGA301.1338128E-437.535691
AGTCGCA301.1338128E-437.535691
TGCACAG301.1345543E-437.531522
CGCTTAG301.1345543E-437.531522
CCGGGCA250.0020976136.034261
CCGGACA250.0020976136.034261
CCATCGG250.0020976136.034261