FastQCFastQC Report
Fri 21 Dec 2018
H5JYVAFXY_n02_Clb2-202-TAP-1_gp.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JYVAFXY_n02_Clb2-202-TAP-1_gp.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1749982
Sequences flagged as poor quality0
Sequence length51
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33840.193373417555152No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG24220.13840142355749943Illumina Single End PCR Primer 1 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGCA502.1827873E-1145.0582161
GTCGCCC2650.044.15624645
GTCGCCG9800.041.56111545
CGGGGCA1600.039.4259381
GCCCTAG301.1315215E-437.548512
CGGGCCA751.8189894E-1236.0465741
CCCCCAA250.002094078836.0465741
CCGCGCA250.002094078836.0465741
TCGCGCA250.002094078836.0465741
CCCGCAA250.002094078836.0465741
TCGGGCA1200.035.6710851
CGGTGGG53300.035.50613845
CGGCCCA458.615789E-735.045281
TGGCCCA458.615789E-735.045281
CGGCTTA458.615789E-735.045281
CGCCGAG653.619789E-1034.6601642
GGTCGCC14550.034.64135444
CCGCGAG606.355549E-933.793662
CCCTAGA801.8189894E-1233.7917253
CCCGGCA551.1155498E-732.769611
Produced by FastQC (version 0.11.5)