FastQCFastQC Report
Fri 21 Dec 2018
H5JYVAFXY_n02_Clb2-202-TAP-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JYVAFXY_n02_Clb2-202-TAP-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2997610
Sequences flagged as poor quality0
Sequence length51
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG66610.22221036092086696Illumina Single End PCR Primer 1 (100% over 51bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41880.1397113033383262No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCGA302.1519245E-645.044681
ACGCCCA253.8695685E-545.044681
ACCGCGA302.1519245E-645.044681
CCGTGAA302.1519245E-645.044681
CCGACCA302.1519245E-645.044681
CACCCTA253.8695685E-545.044681
CCTGGCA453.8198777E-1045.0446781
CTCGCCC207.0365815E-444.9980845
GTCGCCC7150.044.3687445
GTCGCCG20050.043.42707445
CCGGGCA1300.043.312191
ACGCGCA1150.043.0862161
CGGCTCA1000.042.7924461
CTGGCCA1000.042.7924461
TTGCGCA650.041.5797041
CGCGGCA1200.041.290961
ACGGGCA2350.041.2110941
CGGGGCA4500.041.0407071
CGGCCCA1100.040.949711
CGGGCCA1650.040.949711
Produced by FastQC (version 0.11.5)