FastQCFastQC Report
Fri 21 Dec 2018
H5JYVAFXY_n01_wildtype-2_gp.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JYVAFXY_n01_wildtype-2_gp.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3179650
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGA68110.21420596606544748Illumina Single End PCR Primer 1 (96% over 28bp)
CACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCT63070.19835516487663735Illumina Single End PCR Primer 1 (96% over 30bp)
ACACTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTG56540.1778183133363735TruSeq Adapter, Index 5 (97% over 37bp)
CTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTG54620.17177991288349345Illumina Single End PCR Primer 1 (96% over 29bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTC52790.1660245624518422Illumina Single End PCR Primer 1 (96% over 31bp)
CACTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGA52470.1650181623763622TruSeq Adapter, Index 25 (100% over 38bp)
CACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTC51890.16319406223955468Illumina Single End PCR Primer 1 (96% over 31bp)
CTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGAA50810.15979746198480965Illumina Single End PCR Primer 1 (96% over 27bp)
CACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTG47170.14834966112622458Illumina Single End PCR Primer 1 (96% over 29bp)
ACACTCTTTCCCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT46450.14608526095639457TruSeq Adapter, Index 8 (100% over 36bp)
CTCTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGAACT45710.14375796078184705Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACTCTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGAA45440.1429088107181608Illumina Single End PCR Primer 1 (96% over 27bp)
ACACTCTTTCCCTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC45150.14199676064975705TruSeq Adapter, Index 5 (100% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT44110.13872596040444704TruSeq Adapter, Index 25 (97% over 44bp)
CTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGAAC43560.13699621027471579Illumina Single End PCR Primer 1 (96% over 26bp)
CTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATA42770.1345116600883745TruSeq Adapter, Index 25 (100% over 40bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGT42440.13347381001053574Illumina Single End PCR Primer 1 (96% over 32bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCT41550.13067475980060697Illumina Single End PCR Primer 1 (96% over 30bp)
CACTCTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACTC39980.1257371094302832Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACTCCA39470.12413315930998693Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACACTCTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGA38160.12001320900099066Illumina Single End PCR Primer 1 (96% over 28bp)
CACTCTTTCCCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTG37520.11800040885003066TruSeq Adapter, Index 5 (97% over 37bp)
CACTCTTTCCCTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT36480.11472960860472063TruSeq Adapter, Index 8 (100% over 36bp)
ACACTCTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACT36370.11438365857877439Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACACTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGA36300.11416350856226314TruSeq Adapter, Index 25 (100% over 38bp)
CTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTGAA35800.11259100844432564Illumina Single End PCR Primer 1 (96% over 27bp)
TCACTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTG34710.10916295818722185TruSeq Adapter, Index 5 (97% over 37bp)
CTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGAACT33830.10639535797965184Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT33290.10469705785227934TruSeq Adapter, Index 25 (97% over 44bp)
CACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTGA33100.10409950780746308Illumina Single End PCR Primer 1 (96% over 29bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTG32430.10199235764942682Illumina Single End PCR Primer 1 (96% over 30bp)
TTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGAACT32120.10101740757630558Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCTTTCCCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGAT31800.10001100750082557TruSeq Adapter, Index 25 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT17850.036.44546545
GCACTCT9050.034.304251
ACACTCT261250.034.0829281
TGGTCGG2200.031.6997911
GTCTTTC29900.029.7196221
TCTCGTA21550.029.45634544
GACTCTT12200.029.3193741
TCCCGGA2250.028.9956511
GCCCGGA1300.027.6881561
TCGCTCT13000.027.5151021
TCACTCT124250.027.2856461
ATATATG40950.026.55076845
GGCCGCT801.0565418E-725.3087061
GTTTCCC32350.025.173971
GGGCCGA651.5266229E-524.2271371
GCCCCGA1400.024.1035291
TGGGGGG7500.023.99641
TCGCGGA602.4713465E-422.4966241
CACTCTT556950.022.3376032
GCGCGAG1257.566996E-1021.5933672
Produced by FastQC (version 0.11.5)