H5JYVAFXY_n01_wildtype-2_gp_b.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H5JYVAFXY_n01_wildtype-2_gp_b.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3359842
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGA	14066	0.41865063892885435	Illumina Single End PCR Primer 1 (96% over 28bp)
CTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTG	13451	0.400346206756151	Illumina Single End PCR Primer 1 (96% over 29bp)
CTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGAA	11293	0.33611699597778705	Illumina Single End PCR Primer 1 (96% over 27bp)
CTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGAAC	10450	0.31102653041422784	Illumina Single End PCR Primer 1 (96% over 26bp)
CTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTGAA	9659	0.28748375667665327	Illumina Single End PCR Primer 1 (96% over 27bp)
CTCTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGAACT	9556	0.28441813632902974	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCT	9528	0.2835847638073457	Illumina Single End PCR Primer 1 (96% over 30bp)
CTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACACGTCT	9195	0.2736735834601746	Illumina Single End PCR Primer 1 (100% over 30bp)
CACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTC	9167	0.2728402109384906	Illumina Single End PCR Primer 1 (96% over 31bp)
CTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGAACT	8150	0.24257093041875183	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTG	7483	0.2227188064200638	Illumina Single End PCR Primer 1 (96% over 29bp)
CTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTGAAC	7195	0.21414697476845634	Illumina Single End PCR Primer 1 (96% over 27bp)
CTCTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACTCCA	7192	0.2140576848554188	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACACGTCTGA	7067	0.21033727181218642	Illumina Single End PCR Primer 1 (100% over 28bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTC	7028	0.20917650294269788	Illumina Single End PCR Primer 1 (96% over 31bp)
CACTCTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGAA	6821	0.20301549894310508	Illumina Single End PCR Primer 1 (96% over 27bp)
CTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGAACTCC	6763	0.20128922729104526	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGT	6256	0.18619923198769467	Illumina Single End PCR Primer 1 (96% over 32bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCT	5877	0.17491893964061406	Illumina Single End PCR Primer 1 (96% over 30bp)
CACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTGA	5618	0.16721024381503655	Illumina Single End PCR Primer 1 (96% over 29bp)
CTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTGAACTC	5389	0.1603944471198348	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACTCTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACTC	5272	0.15691214051136929	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCTTTCCCTACACGACGCTCTTAGATCGGAAGAGCACACGTCTGAACTCC	5148	0.15322149077248273	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACACTCTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGA	4863	0.1447389490339129	Illumina Single End PCR Primer 1 (96% over 28bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTG	4691	0.13961966068642512	Illumina Single End PCR Primer 1 (96% over 30bp)
CTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT	4618	0.1374469394691774	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCTTTCCCTACACGACGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG	4421	0.13158356851304318	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGAA	4418	0.1314942786000056	Illumina Single End PCR Primer 1 (96% over 27bp)
TTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGAACT	4393	0.13075019599135912	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA	4331	0.12890487112191584	TruSeq Adapter, Index 8 (100% over 40bp)
CTCTTTCCCTACACGACGCTCTTCCTCAGATCGGAAGAGCACACGTCTGAA	4296	0.12786315546981078	Illumina Single End PCR Primer 1 (96% over 25bp)
ACACTCTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACT	4035	0.12009493303554156	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTGAAC	3988	0.11869605773128618	Illumina Single End PCR Primer 1 (96% over 26bp)
CACTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG	3968	0.118100791644369	TruSeq Adapter, Index 8 (100% over 38bp)
CTCTTTCCCTACACGACGCTCTTCCGATCTCAGATCGGAAGAGCACACGTC	3760	0.11191002434043028	Illumina Single End PCR Primer 1 (100% over 30bp)
TCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTGA	3705	0.11027304260140805	Illumina Single End PCR Primer 1 (96% over 28bp)
CCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGAACTCCAG	3678	0.10946943338406984	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CACTCTTTCCCTACACGACGCTCAGATCGGAAGAGCACACGTCTGAACTCC	3667	0.1091420370362654	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCTTTCCCTACACGACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC	3651	0.10866582416673165	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACACTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT	3541	0.10539186068868715	TruSeq Adapter, Index 8 (100% over 37bp)
CACTCTTTCCCTACACGACGCTCTTAGATCGGAAGAGCACACGTCTGAACT	3541	0.10539186068868715	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	3487	0.10378464225401075	No Hit
CTTTCCCTACACGACGCTCTTAGATCGGAAGAGCACACGTCTGAACTCCAG	3485	0.10372511564531904	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCTTTCCCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGA	3452	0.10274292660190569	TruSeq Adapter, Index 8 (100% over 39bp)
CTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGAACTCCAGT	3404	0.10131428799330444	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTG	3400	0.101195234775921	Illumina Single End PCR Primer 1 (96% over 29bp)
CCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTGAACTCCA	3379	0.10057020538465798	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGAACTC	3376	0.1004809154716204	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGTCGG	250	0.0	40.49597	1
GCCTGGA	60	1.5643309E-10	37.49627	1
ACACTCT	24315	0.0	36.566376	1
GTCTTTC	4085	0.0	36.073517	1
GCGGCAA	70	2.1827873E-11	35.35362	1
GCACTCT	790	0.0	35.028156	1
GTGTTTC	510	0.0	34.84947	1
GCCCCGA	185	0.0	34.050663	1
GGCCTTA	40	1.562522E-5	33.74664	1
GACTCTT	1545	0.0	33.346195	1
CGGTTGG	75	4.7293724E-11	33.01145	45
GGGTTGG	150	0.0	33.01145	45
TTGCGCA	110	0.0	32.724014	1
GCCTCGG	50	1.9777563E-6	31.510935	45
AGCCCGA	50	1.9846611E-6	31.496864	1
TATCGGG	170	0.0	30.451744	45
CGGTTGC	30	0.005139922	30.010416	45
GGGGCAA	45	3.4951965E-5	29.997013	1
GGCCGTA	45	3.4951965E-5	29.997013	1
GCCCGTA	105	0.0	29.997013	1

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content