FastQCFastQC Report
Fri 21 Dec 2018
H5JYVAFXY_n01_wildtype-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JYVAFXY_n01_wildtype-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3489423
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGA39630.11357178536394127Illumina Single End PCR Primer 1 (96% over 28bp)
CACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCT38770.11110719451324762Illumina Single End PCR Primer 1 (96% over 30bp)
CTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTG35660.1021945462043438Illumina Single End PCR Primer 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCGA3900.038.0726781
TTGCGCA1550.036.2862781
AGCCGGG250.002104847536.0099245
AGCCGAG2700.035.828312
GCGCCGA1450.035.6856771
GGGCGCA900.034.99611
GCCCCAA3250.034.6115261
TGGCCGA1950.034.6115261
GGCCCAA1700.034.407931
GGTGCGA850.034.407931
TGCCGGA1350.033.3296171
CGGTGAG950.033.1532482
TGCCCGA3100.032.657651
GCCCGAA2900.032.5825731
AGCCCGA900.032.496381
GTCTTTC24400.032.178791
GGGCCGA1400.032.1392751
GCGGCGA1400.032.1392751
CGCGGAG2100.032.1383552
CCCCGAG9750.032.072432
Produced by FastQC (version 0.11.5)