H5JYVAFXY_n01_wildtype-1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H5JYVAFXY_n01_wildtype-1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2347222
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGA	3220	0.1371834449404445	Illumina Single End PCR Primer 1 (96% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	2965	0.1263195385864652	No Hit
CTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTG	2582	0.11000237727833158	Illumina Single End PCR Primer 1 (96% over 29bp)
CACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCT	2510	0.10693492136661978	Illumina Single End PCR Primer 1 (96% over 30bp)
CACTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC	2501	0.1065514893776558	TruSeq Adapter, Index 1 (100% over 38bp)
CTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGAA	2435	0.10373965479191997	Illumina Single End PCR Primer 1 (96% over 27bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	295	0.0	45.01865	45
GGGCGAA	40	6.830305E-9	44.992756	1
GCCGTGA	20	7.0403726E-4	44.992756	1
GCGCCGA	125	0.0	39.593628	1
GGCCTAA	40	3.4646473E-7	39.36866	1
GGCTCGA	75	0.0	38.99372	1
GGCCGTA	35	6.257984E-6	38.56522	1
GCGCCAA	125	0.0	37.793915	1
CGAGGTG	30	1.1375063E-4	37.515545	45
TCGTGCA	30	1.14136536E-4	37.493965	1
GGTCCGA	55	2.755769E-9	36.812256	1
CGTATGC	1630	0.0	36.45682	45
GTGTCGA	50	4.8896254E-8	35.994205	1
GGGGCGA	25	0.0021092205	35.994205	1
GGGCCAA	75	1.8189894E-12	35.9942	1
AGGTAAG	25	0.0021096617	35.992672	2
GTCGAGC	25	0.0021096617	35.992672	3
GCCCCGA	360	0.0	35.619267	1
TGGTCGG	215	0.0	35.57567	1
GGGCCGA	90	0.0	34.99437	1