FastQCFastQC Report
Fri 21 Dec 2018
H5JYVAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JYVAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14748602
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG376810.25548862190463884No Hit
CTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACACGTCT227810.15446209749235892Illumina Single End PCR Primer 1 (100% over 30bp)
CTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTG189850.12872406483000898Illumina Single End PCR Primer 1 (96% over 29bp)
CACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCT167510.11357686647181882Illumina Single End PCR Primer 1 (96% over 30bp)
CACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTC163800.11106137381698958Illumina Single End PCR Primer 1 (96% over 31bp)
CTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGA158520.10748137348882288Illumina Single End PCR Primer 1 (96% over 28bp)
CTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACACGTCTGA149750.10153504718616721Illumina Single End PCR Primer 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT809400.030.021751
GTCTTTC157350.029.6310561
GATCGCG34100.026.14712545
GACTCTT56750.026.0780241
GTTTCCC180950.025.2195931
CATCGGG23600.024.70989645
TATCGGG33650.024.69019145
GCACTCT65400.024.1764321
GATGGGG47000.024.04855545
GATGGCG39100.023.66727345
AATCGCG20450.023.56153745
AATCGGG25150.023.3660745
TATCGCG24250.023.21193945
GATGTCG66300.023.09290145
TCACTCT383700.022.2333891
TATCTCG85200.021.96061745
CCCCGAG27750.021.7998682
CATGGGG23100.021.2484745
TGATGGG86500.020.87571745
GTGTTTC36000.020.741981