FastQCFastQC Report
Fri 21 Dec 2018
H5JYVAFXY_n01_rnh202_pol2-MG-2_gp.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JYVAFXY_n01_rnh202_pol2-MG-2_gp.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3393327
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGA93900.2767195734451764Illumina Single End PCR Primer 1 (96% over 28bp)
CACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCT85700.25255449887381914Illumina Single End PCR Primer 1 (96% over 30bp)
CTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTG83640.246483760627844Illumina Single End PCR Primer 1 (96% over 29bp)
CACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTC76200.22455837589480765Illumina Single End PCR Primer 1 (96% over 31bp)
CTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGAA74410.21928331693349917Illumina Single End PCR Primer 1 (96% over 27bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTC71840.21170962892759818Illumina Single End PCR Primer 1 (96% over 31bp)
CTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGAAC69760.20557995147535146Illumina Single End PCR Primer 1 (96% over 26bp)
CACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTG67930.2001870141014998Illumina Single End PCR Primer 1 (96% over 29bp)
CTCTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGAACT67420.19868406434157393Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACTCTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGAA66260.19526559037782093Illumina Single End PCR Primer 1 (96% over 27bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGT59890.17649345318031537Illumina Single End PCR Primer 1 (96% over 32bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCT59070.17407694572317964Illumina Single End PCR Primer 1 (96% over 30bp)
CTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTGAA58450.17224983032875996Illumina Single End PCR Primer 1 (96% over 27bp)
CTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACACGTCT57240.16868400834932795Illumina Single End PCR Primer 1 (100% over 30bp)
CTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGAACT54020.15919479613959986Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACACTCTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGA53320.15713192392009376Illumina Single End PCR Primer 1 (96% over 28bp)
CACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTGA51460.15165057773683469Illumina Single End PCR Primer 1 (96% over 29bp)
CTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTGAAC48640.14334014965253866Illumina Single End PCR Primer 1 (96% over 27bp)
CTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGAACTCC48620.14328121044626704Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTG48310.1423676527490572Illumina Single End PCR Primer 1 (96% over 30bp)
CTCTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACTCCA45830.1350591911713784Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CACTCTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACTC42580.12548157015224293Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACACGTCTGA42420.1250100565020701Illumina Single End PCR Primer 1 (100% over 28bp)
ACACTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTC41680.12282930587002078TruSeq Adapter, Index 13 (97% over 37bp)
CACTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCC41050.12097272087246529TruSeq Adapter, Index 27 (100% over 38bp)
TTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGAACT38950.11478410421394696Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACACTCTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACT38500.11345797207283588Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGAA37500.11051101175925573Illumina Single End PCR Primer 1 (96% over 27bp)
ACACTCTTTCCCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATT36760.10833026112720642TruSeq Adapter, Index 8 (97% over 36bp)
CTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTGAACTC35890.10576640565439169Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTT35890.10576640565439169TruSeq Adapter, Index 27 (100% over 40bp)
ACACTCTTTCCCTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT35840.10561905763871268TruSeq Adapter, Index 1 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT250600.035.3269581
GTCTTTC35100.030.6382061
TCACTCT111400.029.5865941
CTTTATG36300.028.76870745
TGGTCGG2350.028.7207621
GTTTCCC34900.028.5575451
CGCGTAT2850.028.42932945
GACTCTT14950.027.3887841
GCACTCT10050.026.191621
TCGCGTA3100.026.13664444
TCGCTCT12650.025.9659881
CTCGTAT8050.025.4421945
TGGGGGG9050.024.859591
TTTATCG54350.023.52110745
ATCGCGT4750.022.743843
GTCCGAG802.9826442E-622.495612
CACTCTT561300.021.9224972
TCTCGTA9200.021.03873444
CTTTAGG5650.020.71399545
CGGGTAT1653.6379788E-1220.46050345
Produced by FastQC (version 0.11.5)