FastQCFastQC Report
Fri 21 Dec 2018
H5JYVAFXY_n01_rnh202_pol2-MG-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JYVAFXY_n01_rnh202_pol2-MG-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3440865
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTC124520.3618857467526334Illumina Single End PCR Primer 1 (96% over 31bp)
CACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCT115330.33517734639400265Illumina Single End PCR Primer 1 (96% over 30bp)
CTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTG107920.31364206384150495Illumina Single End PCR Primer 1 (96% over 29bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTC105050.30530113794060504Illumina Single End PCR Primer 1 (96% over 31bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGT103650.30123239359870263Illumina Single End PCR Primer 1 (96% over 32bp)
CTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGA99380.28882272335590037Illumina Single End PCR Primer 1 (96% over 28bp)
CACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTG94950.27594805375973774Illumina Single End PCR Primer 1 (96% over 29bp)
CACTCTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGAA90910.2642068200873908Illumina Single End PCR Primer 1 (96% over 27bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCT89290.259498701634618Illumina Single End PCR Primer 1 (96% over 30bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTG86010.24996621489073242Illumina Single End PCR Primer 1 (96% over 30bp)
CACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTGA83420.242439037858213Illumina Single End PCR Primer 1 (96% over 29bp)
CTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGAA83060.2413927893131524Illumina Single End PCR Primer 1 (96% over 27bp)
ACACTCTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGA81460.23674279577954963Illumina Single End PCR Primer 1 (96% over 28bp)
CTCTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGAACT76840.2233159394512717Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTGAA69530.20207128149462417Illumina Single End PCR Primer 1 (96% over 27bp)
CTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGAAC68770.19986253456616287Illumina Single End PCR Primer 1 (96% over 26bp)
CTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTGAAC64370.18707505234875532Illumina Single End PCR Primer 1 (96% over 27bp)
CTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACACGTCT63860.18559286690991947Illumina Single End PCR Primer 1 (100% over 30bp)
CACTCTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACTC57400.16681851801799838Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGAACT54100.15722790635494271Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACACTCTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACT52810.15347884906847553Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGAACTCC52200.15170603903378946Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGAA48940.1422316772090739Illumina Single End PCR Primer 1 (96% over 27bp)
CTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACACGTCTGA47730.1387151195992868Illumina Single End PCR Primer 1 (100% over 28bp)
CTCTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACTCCA47450.13790137073090633Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCT46290.13453012541904433Illumina Single End PCR Primer 1 (96% over 30bp)
ACACTCTTTCCCTACACGACGCTCAGATCGGAAGAGCACACGTCTGAACTC46280.13450106295945932Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTGA45890.13336762703564364Illumina Single End PCR Primer 1 (96% over 28bp)
TTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGAACT45570.13243762832892308Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTGAACTC44960.13066481829423707Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACTCTTTCCCTACACGACGCTCAGATCGGAAGAGCACACGTCTGAACTCC44950.13063575583465203Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTG44460.1292116953149862Illumina Single End PCR Primer 1 (96% over 29bp)
TTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTGAAC42150.12249826715084725Illumina Single End PCR Primer 1 (96% over 26bp)
CACTCTTTCCCTACACGACGCTCTTAGATCGGAAGAGCACACGTCTGAACT40480.11764483640014937Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACACTCTTTCCCTACACGACGCTCTTAGATCGGAAGAGCACACGTCTGAAC39100.11363421697741702Illumina Single End PCR Primer 1 (96% over 26bp)
TCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTGAACT38240.11113484545310555Illumina Single End PCR Primer 1 (96% over 26bp)
ACACTCTTTCCCTACACGACGCAGATCGGAAGAGCACACGTCTGAACTCCA38240.11113484545310555Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCTTTCCCTACACGACGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG37130.10790891243916866Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGT36720.10671735159618292Illumina Single End PCR Primer 1 (96% over 32bp)
CTCTTTCCCTACACGACGCTCTTAGATCGGAAGAGCACACGTCTGAACTCC36470.10599079010655751Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACACTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC36270.10540954091485717TruSeq Adapter, Index 4 (100% over 37bp)
TTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGAACTC36260.10538047845527214Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGA36070.10482829172315683Illumina Single End PCR Primer 1 (96% over 28bp)
TCTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGAACTC36010.10465391696564673Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC35460.10305548168847078TruSeq Adapter, Index 4 (100% over 38bp)
TTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTGAACTCC34910.10145704641129484Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTGAA34770.1010501719771046Illumina Single End PCR Primer 1 (96% over 28bp)
ACACTCTTTCCCTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG34580.10049798524498926TruSeq Adapter, Index 2 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC12200.036.53003345
CGTATGG5500.035.60420245
ACACTCT367250.033.3987661
GCCCCGA1850.032.8343471
GACTCTT15750.032.1394421
TGGTCGG2650.031.4117571
GTCTTTC36450.029.5030381
CAATCTG11850.029.25142745
TCGTATG20150.028.48375344
TGGGGGG6650.028.4180361
GGCCGGA406.1724987E-428.1220131
TCACTCT142700.027.7318131
TCGCTCT15600.027.6893651
GTGTTTC6100.026.923371
CTCGTAT18600.026.62206843
GTTTCCC40600.026.4873351
GCACTCT9900.026.3608361
AATCTCG110750.025.85163945
GCGTATG2550.025.59704844
TCGCGCA801.0561598E-725.309811
Produced by FastQC (version 0.11.5)