FastQCFastQC Report
Fri 21 Dec 2018
H5JYVAFXY_n01_rnh202_pol2-MG-1_gp.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JYVAFXY_n01_rnh202_pol2-MG-1_gp.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1973975
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGA50260.25461315366202714Illumina Single End PCR Primer 1 (96% over 28bp)
CTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTG45230.22913157461467343Illumina Single End PCR Primer 1 (96% over 29bp)
CTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGAA39340.19929330411985968Illumina Single End PCR Primer 1 (96% over 27bp)
CTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGAAC37100.1879456426753125Illumina Single End PCR Primer 1 (96% over 26bp)
CTCTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGAACT36200.18338631441634268Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCT32870.16651679985815424Illumina Single End PCR Primer 1 (96% over 30bp)
CTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTGAA32450.16438911333730163Illumina Single End PCR Primer 1 (96% over 27bp)
CTCTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACTCCA30370.15385199913879355Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACACGTCT29640.15015387732874025Illumina Single End PCR Primer 1 (100% over 30bp)
CACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTC29030.1470636659532162Illumina Single End PCR Primer 1 (96% over 31bp)
CTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGAACT28960.14670905153307412Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTG25860.1310046986410669Illumina Single End PCR Primer 1 (96% over 29bp)
CTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGAACTCC25280.12806646487417522Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTC24910.12619207436770982Illumina Single End PCR Primer 1 (96% over 31bp)
CTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTGAAC24810.1256854823389354Illumina Single End PCR Primer 1 (96% over 27bp)
CACTCTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGAA24760.1254321863245482Illumina Single End PCR Primer 1 (96% over 27bp)
CTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACACGTCTGA23890.12102483567421067Illumina Single End PCR Primer 1 (100% over 28bp)
CTCTTTCCCTACACGACGCTCTTAGATCGGAAGAGCACACGTCTGAACTCC21960.11124760951886421Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGT20710.10491520915918387Illumina Single End PCR Primer 1 (96% over 32bp)
CACTCTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACTC20130.1019769753922922Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTTC19900.034.936931
ACACTCT90500.034.6820641
CGTATGC15100.033.8345445
AGCCGAG1150.031.2985692
CGTATGG1600.029.54001445
GTTTCCC22550.029.5341531
TGGGGGG5050.029.4057251
GATCGCG5150.028.8435145
TGGTCGG1800.028.749791
GACTCTT6600.028.2952461
TCACTCT43850.026.9895421
TCGTATG20350.025.9905144
GGCACAG1159.094947E-1225.4300862
GCTGGGG450.00122474625.00742145
GGCCGCT450.00122691924.9998171
GATCTCG157350.024.73088545
CCCCGAG3200.024.6048322
CGCGGAG751.7014081E-623.995572
GCCCGTA851.8952232E-723.8233571
CCCGGAG851.898461E-723.8191322
Produced by FastQC (version 0.11.5)