FastQCFastQC Report
Fri 21 Dec 2018
H5JYVAFXY_n01_rnh202_pol2-MG-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JYVAFXY_n01_rnh202_pol2-MG-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1484749
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18040.12150201818623889No Hit
CTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGA16380.11032167726666257Illumina Single End PCR Primer 1 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGG1250.032.396461
ACACTCT60450.032.0064281
GTCTTTC11800.028.7892271
GGCCCGA856.491973E-926.4676951
TCACTCT36050.026.2107281
GCCCGTA801.0553413E-725.3097341
TATCGCG16700.025.06474145
TGGGGGG4850.024.5849441
TCGCGGG1101.5097612E-1024.55021345
GGCCTAG551.3694807E-424.5427721
TATCGGG34150.024.38247145
GTATCGG77150.024.26912545
CTCGGGG651.5218535E-524.23546645
GCCCCTA1301.8189894E-1224.228121
CGCGAGA2900.024.0474763
GTTTCCC13050.023.9628981
GCCCGAA851.8964238E-723.8209271
TATCTCG18250.023.79913545
GCGAGAT7100.023.764214
CGGGGAT952.1122105E-823.68880343
Produced by FastQC (version 0.11.5)