FastQCFastQC Report
Fri 21 Dec 2018
H5JYVAFXY_n01_ds774-041-2_ctrl.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JYVAFXY_n01_ds774-041-2_ctrl.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8352289
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGG21950.024.1900631
CGTGTGC15750.017.1419491
CCTTGTA54450.016.24279634
AGTCACC62450.015.854529
GTCACCT61550.015.72167230
TCCGATC74350.015.067655
CCGATCT74400.014.90634256
CAGTCAC72750.014.19733428
TCTTCCG86050.014.0909472
CTTCCGA82100.014.0015093
GTGTGCT20000.013.9474242
TTCCGAT83350.013.8185184
GGTCGGA6350.013.8163462
CACCTTG68800.013.214437532
ACCTTGT67400.012.95486233
TGTAATG38800.012.93504337
CGATCTG34200.012.2341427
TCCAGTC102600.011.3596626
CCAGTCA94400.011.25061727
CTTGTAA74950.011.16986335