H5JYVAFXY_n01_Clb2-202-TAP-2_gp.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H5JYVAFXY_n01_Clb2-202-TAP-2_gp.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1802495
Sequences flagged as poor quality	0
Sequence length	51
%GC	53

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTG	8683	0.4817211698229399	Illumina Single End PCR Primer 1 (96% over 29bp)
CTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGA	8500	0.47156857577968314	Illumina Single End PCR Primer 1 (96% over 28bp)
CACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCT	6948	0.38546570170791045	Illumina Single End PCR Primer 1 (96% over 30bp)
CTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGAA	6826	0.3786973056790726	Illumina Single End PCR Primer 1 (96% over 27bp)
CACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTC	6791	0.37675555271997985	Illumina Single End PCR Primer 1 (96% over 31bp)
CTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACACGTCT	6272	0.3479621302694321	Illumina Single End PCR Primer 1 (100% over 30bp)
CTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGAAC	5920	0.3284336433665558	Illumina Single End PCR Primer 1 (96% over 26bp)
CTCTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGAACT	5808	0.3222200338974588	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTGAA	5570	0.30901611377562765	Illumina Single End PCR Primer 1 (96% over 27bp)
CACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTG	5347	0.2966443734934078	Illumina Single End PCR Primer 1 (96% over 29bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTC	5303	0.2942033126305482	Illumina Single End PCR Primer 1 (96% over 31bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGT	4781	0.2652434542120783	Illumina Single End PCR Primer 1 (96% over 32bp)
CACTCTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGAA	4721	0.2619147348536334	Illumina Single End PCR Primer 1 (96% over 27bp)
CTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGAACT	4640	0.25742096371973294	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACACGTCTGA	4272	0.23700481832127138	Illumina Single End PCR Primer 1 (100% over 28bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCT	4265	0.23661646772945277	Illumina Single End PCR Primer 1 (96% over 30bp)
CTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTGAAC	4243	0.23539593729802302	Illumina Single End PCR Primer 1 (96% over 27bp)
CACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTGA	4073	0.22596456578242935	Illumina Single End PCR Primer 1 (96% over 29bp)
CTCTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACTCCA	4004	0.2221365385202178	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGAACTCC	3794	0.21048602076566095	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTG	3522	0.19539582634071107	Illumina Single End PCR Primer 1 (96% over 30bp)
ACACTCTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGA	3476	0.19284380816590338	Illumina Single End PCR Primer 1 (96% over 28bp)
CACTCTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACTC	3217	0.17847483626861657	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTGAACTC	2991	0.1659366600184744	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCTTTCCCTACACGACGCTCTTAGATCGGAAGAGCACACGTCTGAACTCC	2976	0.1651044801788632	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCTTTCCCTACACGACGCTCTTCCTCAGATCGGAAGAGCACACGTCTGAA	2694	0.14945949919417253	Illumina Single End PCR Primer 1 (96% over 25bp)
TCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGAA	2474	0.13725419487987484	Illumina Single End PCR Primer 1 (96% over 27bp)
TTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGAACT	2473	0.13719871622390076	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACACTCTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACT	2468	0.13692132294403037	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCTTTCCCTACACGACGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG	2437	0.13520148460883386	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT	2412	0.13381451820948187	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCT	2369	0.13142893600259642	Illumina Single End PCR Primer 1 (96% over 30bp)
TTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTGAAC	2324	0.12893239648376278	Illumina Single End PCR Primer 1 (96% over 26bp)
ACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTG	2323	0.1288769178277887	Illumina Single End PCR Primer 1 (96% over 29bp)
TCACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGT	2317	0.12854404589194424	Illumina Single End PCR Primer 1 (96% over 32bp)
CTCTTTCCCTACACGACGCTCTTCCGATCTCAGATCGGAAGAGCACACGTC	2314	0.12837760992402197	Illumina Single End PCR Primer 1 (100% over 30bp)
CACTCTTTCCCTACACGACGCTCAGATCGGAAGAGCACACGTCTGAACTCC	2292	0.1271570794925922	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACTCTTTCCCTACACGACGCTCTTAGATCGGAAGAGCACACGTCTGAACT	2287	0.1268796862127218	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACTCTTTCCCTACACGACGCTCTTCCTCAGATCGGAAGAGCACACGTCTG	2153	0.11944554631219503	Illumina Single End PCR Primer 1 (96% over 27bp)
TCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTGA	2150	0.1192791103442728	Illumina Single End PCR Primer 1 (96% over 28bp)
CCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGAACTCCAG	2131	0.11822501588076527	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCTTTCCCTACACGACGCTCTTCCGTAGATCGGAAGAGCACACGTCTGAA	2059	0.11423055265063148	Illumina Single End PCR Primer 1 (96% over 26bp)
CCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTGAACTCCA	2037	0.11301002221920171	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGAACTCCAGT	1991	0.11045800404439403	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTGAACTCCAG	1989	0.11034704673244587	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCTTTCCCTACACGACGCTCTTCCGATCTGAGATCGGAAGAGCACACGTC	1967	0.10912651630101608	Illumina Single End PCR Primer 1 (100% over 30bp)
CTTTCCCTACACGACGCTCTTAGATCGGAAGAGCACACGTCTGAACTCCAG	1934	0.10729572065387144	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGAACTC	1920	0.10651901947023432	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACACTCTTTCCCTACACGACGCTCAGATCGGAAGAGCACACGTCTGAACTC	1892	0.10496561710296005	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCTTTCCCTACACGACGCTCTTCAGATCGGAAGAGCACACGTCTGAACTC	1874	0.10396700129542663	Illumina Single End PCR Primer 1 (96% over 29bp)
ACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGA	1841	0.10213620564828195	Illumina Single End PCR Primer 1 (96% over 28bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCGA	25	3.891903E-5	44.999588	1
GGCCCAA	25	3.891903E-5	44.999588	1
TATGGAG	20	7.0346746E-4	44.999584	1
TGTGACA	20	7.037559E-4	44.99584	2
ATGGTAG	20	7.037559E-4	44.99584	2
GAGTCTT	55	6.184564E-11	40.908714	1
CGTATGG	160	0.0	40.787663	45
CGTTTGG	50	1.0804797E-9	40.50637	45
GGGCCGA	50	1.0822987E-9	40.499626	1
TGGTCGG	90	0.0	39.99963	1
GCACTCT	500	0.0	39.599632	1
GTCGTTT	40	3.454661E-7	39.382286	43
TCGCGCT	40	3.459936E-7	39.374638	1
GTGTTTC	310	0.0	37.741585	1
GCCCCAA	90	0.0	37.499653	1
GCCCGGA	30	1.1402412E-4	37.499653	1
CGTATGC	370	0.0	37.100426	45
GTCTTTC	3015	0.0	36.641453	1
GACTCTT	985	0.0	36.31946	1
CGACATG	50	4.8736183E-8	36.00666	43

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content