H5JYVAFXY_n01_Clb2-202-TAP-2.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H5JYVAFXY_n01_Clb2-202-TAP-2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4050410
Sequences flagged as poor quality	0
Sequence length	51
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTC	15750	0.38884952387536076	Illumina Single End PCR Primer 1 (96% over 31bp)
CTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTG	15372	0.3795171353023521	Illumina Single End PCR Primer 1 (96% over 29bp)
CACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCT	13828	0.34139753753323737	Illumina Single End PCR Primer 1 (96% over 30bp)
CTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGA	13226	0.32653484462066806	Illumina Single End PCR Primer 1 (96% over 28bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGT	11766	0.2904891109788886	Illumina Single End PCR Primer 1 (96% over 32bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTC	11331	0.279749457462331	Illumina Single End PCR Primer 1 (96% over 31bp)
CTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACACGTCT	11207	0.2766880389886456	Illumina Single End PCR Primer 1 (100% over 30bp)
CACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTG	10658	0.2631338555849902	Illumina Single End PCR Primer 1 (96% over 29bp)
CTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGAA	10340	0.2552827985315067	Illumina Single End PCR Primer 1 (96% over 27bp)
CACTCTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGAA	10181	0.25135727000476493	Illumina Single End PCR Primer 1 (96% over 27bp)
CTCTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGAACT	9456	0.23345784747716897	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTGAA	9298	0.229557007809086	Illumina Single End PCR Primer 1 (96% over 27bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCT	8945	0.22084184070254617	Illumina Single End PCR Primer 1 (96% over 30bp)
CACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTGA	8897	0.219656775486926	Illumina Single End PCR Primer 1 (96% over 29bp)
CTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGAAC	8611	0.21259576191052262	Illumina Single End PCR Primer 1 (96% over 26bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTG	8117	0.20039946573309864	Illumina Single End PCR Primer 1 (96% over 30bp)
ACACTCTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGA	7861	0.19407911791645785	Illumina Single End PCR Primer 1 (96% over 28bp)
CTCTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACTCCA	7431	0.18346290869319404	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CACTCTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACTC	7326	0.18087057853402494	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTGAAC	7317	0.1806483788060962	Illumina Single End PCR Primer 1 (96% over 27bp)
CTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACACGTCTGA	7208	0.1779572932122921	Illumina Single End PCR Primer 1 (100% over 28bp)
CTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGAACT	6679	0.16489688698181174	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACTCTTTCCCTACACGACGCTCAGATCGGAAGAGCACACGTCTGAACTCC	6111	0.15087361526363996	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACACTCTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACT	6064	0.1497132389066786	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGAACTCC	6047	0.14929352830947978	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGT	5552	0.13707254327339702	Illumina Single End PCR Primer 1 (96% over 32bp)
CTCTTTCCCTACACGACGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG	5487	0.13546776746057806	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCT	5379	0.13280137072543274	Illumina Single End PCR Primer 1 (96% over 30bp)
TCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTGA	5372	0.13262854871482146	Illumina Single End PCR Primer 1 (96% over 28bp)
TCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGAA	5313	0.13117190605395504	Illumina Single End PCR Primer 1 (96% over 27bp)
TTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGAACT	5306	0.13099908404334376	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTGAAC	5253	0.12969057453442986	Illumina Single End PCR Primer 1 (96% over 26bp)
ACACTCTTTCCCTACACGACGCTCAGATCGGAAGAGCACACGTCTGAACTC	5223	0.12894990877466725	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCTTTCCCTACACGACGCTCTTAGATCGGAAGAGCACACGTCTGAACTCC	5080	0.12541940198646556	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTGAACTC	5022	0.12398744818425789	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACTCTTTCCCTACACGACGCTCTTAGATCGGAAGAGCACACGTCTGAACT	4994	0.1232961601418128	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACACTCTTTCCCTACACGACGCAGATCGGAAGAGCACACGTCTGAACTCCA	4931	0.12174076204631136	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTG	4826	0.11914843188714228	Illumina Single End PCR Primer 1 (96% over 29bp)
CACTCTTTCCCTACACGACGCAGATCGGAAGAGCACACGTCTGAACTCCAG	4693	0.11586481368552815	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT	4408	0.1088284889677835	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CACTCTTTCCCTACACGACGCTCTTCCTCAGATCGGAAGAGCACACGTCTG	4059	0.10021207729587868	Illumina Single End PCR Primer 1 (96% over 27bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGGCG	20	7.0223236E-4	45.016994	45
GGCCGTA	20	7.0372917E-4	44.997543	1
GTGTCCA	20	7.0372917E-4	44.997543	1
ATGCTAG	20	7.040716E-4	44.9931	2
TGCGGGA	60	3.6379788E-12	41.24775	1
CGTTTGG	110	0.0	40.92454	45
CGTATGG	360	0.0	40.640347	45
GCGTTTG	50	1.0786607E-9	40.514793	44
GCGCGAA	50	1.0841177E-9	40.497787	1
GGTGCGA	45	1.931221E-8	39.997818	1
GGGTCAA	35	6.2545387E-6	38.569324	1
GTATGCC	125	0.0	37.814278	45
CGTATTG	60	1.546141E-10	37.514164	45
CGGTTGG	30	1.13789865E-4	37.514164	45
GCACTCT	1330	0.0	37.385174	1
GCCCTCT	170	0.0	37.0568	1
GGCCCGA	185	0.0	36.484493	1
GGTCCAA	25	0.0021083	35.998035	1
GCGGCTA	50	4.886533E-8	35.998035	1
GCCCGCT	25	0.0021083	35.998035	1

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content