FastQCFastQC Report
Fri 21 Dec 2018
H5JYVAFXY_n01_Clb2-202-TAP-1_gp.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JYVAFXY_n01_Clb2-202-TAP-1_gp.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1749982
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGA29380.1678874411279659Illumina Single End PCR Primer 1 (96% over 28bp)
CTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTG25550.14600150172973209Illumina Single End PCR Primer 1 (96% over 29bp)
CTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGAA23820.13611568576133926Illumina Single End PCR Primer 1 (96% over 27bp)
CTCTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGAACT23130.13217278806296293Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCT21850.12485842711525033Illumina Single End PCR Primer 1 (96% over 30bp)
CTCTTTCCCTACACGACGCTCTAGATCGGAAGAGCACACGTCTGAACTCCA21330.12188696798024208Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGAAC20670.11811550061657777Illumina Single End PCR Primer 1 (96% over 26bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19090.10908683632174503No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCCGA850.039.7008631
GCCCCGA1850.037.697941
GCCGCGA850.037.054141
GTCTCGG250.002103988836.01191345
GCCGTGA458.711995E-734.995581
GGCCCGA1100.034.7683331
TGGTCGG1550.033.3828771
GCCGGGA551.12953785E-732.7231371
GCGCCGA1100.032.7231371
GGCCCAG1550.031.9287132
GTCGCGA501.9843574E-631.496021
TGGCGCA501.9843574E-631.496021
CGCCGAG2150.031.3886892
GCCCGTA801.0004442E-1030.9335921
GTCTTTC12750.030.878451
CCGCGAG2200.030.6753122
AATCGGG1250.030.6101345
ACACTCT86300.030.0570371
GCGCGAA453.49489E-529.996211
GCGCCTA453.49489E-529.996211
Produced by FastQC (version 0.11.5)