FastQCFastQC Report
Fri 21 Dec 2018
H5JYVAFXY_n01_Clb2-202-TAP-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JYVAFXY_n01_Clb2-202-TAP-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2997610
Sequences flagged as poor quality0
Sequence length51
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35020.11682640503601202No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33890.11305673519904191No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCAA1900.041.4490281
GCCCCGA5300.041.1808931
TTGGGCA1350.040.0016021
TTTGCGA1700.039.7074741
GCCCGGA1700.039.7074741
CATCGGG800.039.3838145
GCGTGGA403.4596087E-739.3765751
TGCCCGA4650.039.1951181
GCCCCAA6100.039.0999261
GCGCCAA2650.039.0581671
GGGACGA700.038.572971
CAGGCTA356.249933E-638.572971
CGGTCCA356.249933E-638.572971
GGCCCGA2950.038.137121
TCCGGGA659.094947E-1238.078451
GCGCCGA1950.038.0784451
TAGCCGA1950.038.0784451
GGCCCCA950.037.896251
TCGGCGA950.037.896251
GCCTCGA1250.037.8015141
Produced by FastQC (version 0.11.5)