FastQCFastQC Report
Wed 10 Jan 2018
H5JWNBCX2_l02n02_3033_d5.352000000b7a52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JWNBCX2_l02n02_3033_d5.352000000b7a52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14393414
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG699930.4862849078057506No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC424620.2950099260675751No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC324970.2257768726724598No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC298820.20760884109913047No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC261430.18163168237917704No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT250020.1737044456582712No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA243070.1688758483567554No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT234600.162991212508721No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA223650.15538356640057738No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA207030.14383661860903882No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC163170.1133643484443649No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC158620.11020318042682578No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA157770.10961263255541737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT140850.047.9217261
AACCGTG148800.045.482852
ACAACCG67500.043.8656772
ACCGTGT183250.037.0360643
CCGTGTT199100.034.0621574
CGTGTTC216750.031.256875
CACAACC99500.030.8132571
ACAGACT120900.027.6356452
TGTTCAC253600.026.9945227
TTCACTA250550.026.9249489
GTGTTCA260100.026.483576
GTTCACT260500.025.951248
AGACTCA137100.025.2355844
CACAGAC161250.021.8138831
AACTCAC227850.020.580375
ACTCACC230450.020.573246
CAGACTC173300.020.3491333
GAACTCA258200.018.5718154
AGAACTC261150.018.3083213
TCACCAT298700.016.1909948
Produced by FastQC (version 0.11.2)